Can I show the failure rate at each stage?

Add a side-bar funnel showing candidate count at each stage (e.g., 10000 -> 200 -> 20 -> 5 -> 2 -> 1). Place it to the right of the main pipeline.

PromptsPipeline

PipelineText → ImageMid creditEN

Therapeutic siRNA Design Pipeline

End-to-end siRNA discovery from target nomination to LNP-formulated in vivo delivery.

When to use this prompt

For RNA therapeutics, drug-discovery proposals and translational pipeline figures.

The prompt

A therapeutic siRNA design pipeline, six stages arranged left-to-right with thin black arrows between rounded rectangle stages.

Stage 1 — Target Nomination
- Disease-relevant gene shortlisted from omics data and literature.

Stage 2 — siRNA Sequence Design
- Computational design of candidate antisense and sense strands.
- Off-target screen against the transcriptome.

Stage 3 — Chemical Modification
- Backbone chemistries (2'-OMe, 2'-F, phosphorothioate) applied to candidates.
- Conjugation handle (e.g., GalNAc, lipid) added for delivery.

Stage 4 — In Vitro Screen
- Knockdown assay in disease-relevant cell line.
- Lead candidates ranked by IC50 and persistence.

Stage 5 — LNP Formulation
- Lead siRNA encapsulated in lipid nanoparticles for systemic delivery.

Stage 6 — In Vivo Validation
- Mouse model dosed; tissue biodistribution and target knockdown measured.

Annotate each stage with a 2-3 word output label (candidates / leads / formulation / response).
Style: biomedical illustration, navy / teal / coral palette, white background, suitable for grant figures or translational reviews.

Variations

Add a non-human-primate stage

Insert a Stage 5b "NHP Pharmacokinetics" between LNP Formulation and In Vivo Validation, showing biodistribution and immune response checks before mouse efficacy work.

Tips

Number stages 1-6 — readers parse pipelines top-down by number first.
Annotate each stage's output, not just its action. "Knockdown leads" beats "screen".
Keep arrow labels short. Pipeline figures fail when arrows carry sentences.